mouse anti zag  (Novus Biologicals)

Journal: Microbiome

Article Title: Novel bacterial proteolytic and metabolic activity associated with dental erosion-induced oral dysbiosis

doi: 10.1186/s40168-023-01514-0

Figure Lengend Snippet: Immunoblots for ( A ) PIP and ( B ) ZAG proteins identified in saliva samples (E = erosion, H = healthy controls, M = protein marker)

Article Snippet: Primary antibodies were prepared in TBST plus 5% skim milk powder (Sigma-Aldrich) (rabbit anti-PIP 1:2000, mouse anti-ZAG 1:2500), and the membranes were incubated at room temperature on a rotary rocker for one hour.

Techniques: Western Blot, Marker

Journal: Oncology letters

Article Title: Knockdown of cathepsin L sensitizes ovarian cancer cells to chemotherapy.

doi: 10.3892/ol.2016.4494

Figure Lengend Snippet: Figure 1. Western blots showing (A) expression of CTSL in SKOV3 and SKOV3/TAX cell lines; and (B) the effect of Tax treatment on the expression of CTSL in SKOV3 and SKOV3/TAX cell lines. CTSL, cathepsin L; Tax, Taxol (paclitaxel).

Article Snippet: The non-specific binding sites were blocked by immersing the membrane into 5% non-fat milk in Tris-buffered saline with 0.5% Tween-20 (TBS-T) solution (Beijing Solarbio Science and Technology, Co., Ltd.) for 1 h, and then incubating the membrane with a primary mouse monoclonal anti-CTSL antibody (catalog no., sc-135859; dilution, 1:1,000; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) for 2 h at room temperature (RT).

Techniques: Western Blot, Expressing

Journal: Oncology letters

Article Title: Knockdown of cathepsin L sensitizes ovarian cancer cells to chemotherapy.

doi: 10.3892/ol.2016.4494

Figure Lengend Snippet: Figure 2. Western blots showing (A) significantly decreased expression levels of CTSL in SKOV3‑CTSL‑shRNA cells compared with Con cells (SKOV3‑Con‑shRNA); and (B) significantly decreased expression levels of CTSL in SKOV3/TAX‑CTSL‑shRNA cells compared with Con cells (SKOV3/TAX‑Con‑shRNA). Con, control; CTSL, cathespin L; shRNA, small hairpin RNA.

Article Snippet: The non-specific binding sites were blocked by immersing the membrane into 5% non-fat milk in Tris-buffered saline with 0.5% Tween-20 (TBS-T) solution (Beijing Solarbio Science and Technology, Co., Ltd.) for 1 h, and then incubating the membrane with a primary mouse monoclonal anti-CTSL antibody (catalog no., sc-135859; dilution, 1:1,000; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) for 2 h at room temperature (RT).

Techniques: Western Blot, Expressing, Control, shRNA

Journal: Oncology letters

Article Title: Knockdown of cathepsin L sensitizes ovarian cancer cells to chemotherapy.

doi: 10.3892/ol.2016.4494

Figure Lengend Snippet: Figure 3. Knockdown of CTSL in (A) SKOV3 and (B) SKOV3/TAX cells causes decreased cell proliferation compared with Con cells. Cells were counted every day for 3 days by reading the absorbance at 490 nm. Con, control; CTSL, cathespin L; shRNA, small hairpin RNA.

Article Snippet: The non-specific binding sites were blocked by immersing the membrane into 5% non-fat milk in Tris-buffered saline with 0.5% Tween-20 (TBS-T) solution (Beijing Solarbio Science and Technology, Co., Ltd.) for 1 h, and then incubating the membrane with a primary mouse monoclonal anti-CTSL antibody (catalog no., sc-135859; dilution, 1:1,000; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) for 2 h at room temperature (RT).

Techniques: Knockdown, Control, shRNA

Journal: Oncology letters

Article Title: Knockdown of cathepsin L sensitizes ovarian cancer cells to chemotherapy.

doi: 10.3892/ol.2016.4494

Figure Lengend Snippet: Figure 4. (A) Tax induced significantly potentiated apoptosis in SKOV‑3 cells transfected with CTSL‑ or con‑shRNA. *P<0.05 vs. no Tax treatment. (B) CTSL silencing significantly potentiated apoptosis induced by Tax in SKOV3/TAX cells compared with SKOV3/TAX cells transfected with Con‑shRNA. *P<0.05 vs. SKOV3/TAX‑Con‑shRNA cells treated with Tax. Tax, Taxol (paclitaxel); Con, control; shRNA, small hairpin RNA; CTSL, cathepsin L.

Article Snippet: The non-specific binding sites were blocked by immersing the membrane into 5% non-fat milk in Tris-buffered saline with 0.5% Tween-20 (TBS-T) solution (Beijing Solarbio Science and Technology, Co., Ltd.) for 1 h, and then incubating the membrane with a primary mouse monoclonal anti-CTSL antibody (catalog no., sc-135859; dilution, 1:1,000; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) for 2 h at room temperature (RT).

Techniques: Transfection, Control, shRNA